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rabbit α cdk6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit α cdk6
    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing <t>CDK6(D104S)</t> and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.
    Rabbit α Cdk6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 327 article reviews
    rabbit α cdk6 - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species"

    Article Title: HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species

    Journal: Science signaling

    doi: 10.1126/scisignal.aay0482

    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing CDK6(D104S) and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.
    Figure Legend Snippet: (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing CDK6(D104S) and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.

    Techniques Used: Stable Transfection, Infection, Expressing, Western Blot, CRISPR, Control, Mutagenesis



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    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing <t>CDK6(D104S)</t> and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.
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    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing <t>CDK6(D104S)</t> and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.
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    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing <t>CDK6(D104S)</t> and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.
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    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing <t>CDK6(D104S)</t> and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.
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    Image Search Results


    (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing CDK6(D104S) and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.

    Journal: Science signaling

    Article Title: HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species

    doi: 10.1126/scisignal.aay0482

    Figure Lengend Snippet: (A) Ratio of 786-O cells stably infected with a bicistronic lentivirus expressing VHL and Tdtomato (VHL-Tdtomato) to 786-O cells infected with GFP alone (EV-GFP) that had been mixed (1:1) and then treated with 0, 200, or 400 nM palbociclib for 3 to 10 days. Data are means ± SD of n = 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by two-way ANOVA. (B) Immunoblot of total and Ser780-, Ser608-, Ser795-, and Ser807/811-phosphorylated pRb in 786-O cells expressing VHL-Tdtomato or EV-GFP and treated with 100, 200, 400, or 800 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates. (C) 786-O cells that underwent CRISPR/Cas9 editing with an RB1 sgRNA and then were infected, mixed, and treated as in (A). The ratio of RB1-null VHL-Tdtomato cells to RB1-null EV-GFP cells after treatment is shown. Data are means ± SD of n = 3 independent experiments. (D) Immunoblot of Rb, VHL, and actin (loading control) abundance in 786-O cells edited with an RB1 sgRNA (as indicated, +) and infected as described in (C), but not otherwise treated. Blots are representative of three biological replicates. (E) Ratio of 786-O cells stably expressing CDK6(D104S) and either VHL-Tdtomato or EV-GFP that had been mixed and treated as described in (A). Data are means ± SD of n = 3 experiments. (F) Immunoblot of 786-O cells stably infected with lentivirus expressing either WT or mutant (D104S) CDK6 and then treated with 50, 100, 200, 400, 800, or 1600 nM palbociclib, as indicated by the triangle, for 24 hours. Blots are representative of three biological replicates.

    Article Snippet: The primary antibodies used were rabbit a-VHL (1:500; Cell Signaling, no. 68547), rabbit α–HIF-2α (1:1000; Bethyl, no. 118-1261), mouse α-vinculin (1:10,000; Sigma, no. V9131), rabbit α-actin (1:2000; Cell Signaling, no. 4970), rabbit α-tubulin (1:1000; Cell Signaling, no. 2146), rabbit α-CDK4 (1:1000; Cell Signaling, #12790), rabbit α-CDK6 (1:1000; Cell Signaling, no. 13331), rabbit α–phospho-pRb (Ser 780 ) (1:5000; Cell Signaling, no. 8180), α–phospho-pRb (Ser 795 ) (used at 1:5000; Cell Signaling, no. 9301), α–phospho-pRb (Ser 608 ) (1:5000; Cell Signaling, no. 8147), ––phospho-pRb (Ser 807/811 ) (1:5000; Cell Signaling, no. 8516), mouse α-pRb (used at 1:5000; Cell Signaling, no. 9309), rabbit α-NDRG1 (1:750; Cell Signaling, no. 5196), and rabbit α–cyclin D1 (1:500; Cell Signaling, no. 2978).

    Techniques: Stable Transfection, Infection, Expressing, Western Blot, CRISPR, Control, Mutagenesis